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Rothmans Inc ssgfpkdel plasmid
Ssgfpkdel Plasmid, supplied by Rothmans Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssgfpkdel+plasmid/ssgfpkdel+plasmid/pm40593538-395-20-27
Average 90 stars, based on 1 article reviews
ssgfpkdel plasmid - by Bioz Stars, 2026-10
90/100 stars

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Cloning:

Article Title: TUG protein acts through a disordered region to organize the early secretory pathway.
Article Snippet: Cloning of the FUS IDR tagged to mCherry was achieved by PCR amplifying a fragment containing the 214 residues corresponding to the FUS IDR from the pcDNA 3.2-FUS-1-526aa-V5, a gift from Dr. Aaron Gitler (Addgene plasmid # 29609; http://n2t.net/addgene:29609; RRID: Addgene_29609) and then inserted into pB vector along with a C-terminal mCherry tag and a linker that was the same used for cloning of IDR1 from TUG. .. Cloning of the ssGFP plasmid to monitor GFP secretion was achieved by amplifying the signal sequence and EGFP from a ssGFPKDEL plasmid (a gift from Dr. James Rothman’s laboratory). .. For cloning, all PCR amplifications were done using Phusion HighFidelity DNA polymerase, which was obtained from Thermo Fischer Scientific.

Plasmid Preparation:

Article Title: TUG protein acts through a disordered region to organize the early secretory pathway.
Article Snippet: Cloning of the FUS IDR tagged to mCherry was achieved by PCR amplifying a fragment containing the 214 residues corresponding to the FUS IDR from the pcDNA 3.2-FUS-1-526aa-V5, a gift from Dr. Aaron Gitler (Addgene plasmid # 29609; http://n2t.net/addgene:29609; RRID: Addgene_29609) and then inserted into pB vector along with a C-terminal mCherry tag and a linker that was the same used for cloning of IDR1 from TUG. .. Cloning of the ssGFP plasmid to monitor GFP secretion was achieved by amplifying the signal sequence and EGFP from a ssGFPKDEL plasmid (a gift from Dr. James Rothman’s laboratory). .. For cloning, all PCR amplifications were done using Phusion HighFidelity DNA polymerase, which was obtained from Thermo Fischer Scientific.

Sequencing:

Article Title: TUG protein acts through a disordered region to organize the early secretory pathway.
Article Snippet: Cloning of the FUS IDR tagged to mCherry was achieved by PCR amplifying a fragment containing the 214 residues corresponding to the FUS IDR from the pcDNA 3.2-FUS-1-526aa-V5, a gift from Dr. Aaron Gitler (Addgene plasmid # 29609; http://n2t.net/addgene:29609; RRID: Addgene_29609) and then inserted into pB vector along with a C-terminal mCherry tag and a linker that was the same used for cloning of IDR1 from TUG. .. Cloning of the ssGFP plasmid to monitor GFP secretion was achieved by amplifying the signal sequence and EGFP from a ssGFPKDEL plasmid (a gift from Dr. James Rothman’s laboratory). .. For cloning, all PCR amplifications were done using Phusion HighFidelity DNA polymerase, which was obtained from Thermo Fischer Scientific.



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Rothmans Inc ssgfpkdel plasmid
Ssgfpkdel Plasmid, supplied by Rothmans Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssgfpkdel+plasmid/ssgfpkdel+plasmid/pm40593538-395-20-27
Average 90 stars, based on 1 article reviews
ssgfpkdel plasmid - by Bioz Stars, 2026-10
90/100 stars
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